Format for Individual Target pages (copy this list to new Target page and then fill in for your target):
*Target (protein/gene name): *NCBI Gene # or RefSeq#: *Protein ID (NP or XP #) or Wolbachia#: *Organism: Etiologic Risk Group (see link below): *Background/Disease Information: Essentiality of this protein: Complex of enzymes: *EC#: Link to BRENDA EC# page: --Show screenshot of BRENDA enzyme mechanism schematic Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): -- link to Sigma (or other company) page for assay or assay reagents (substrates) -- link to paper that contains assay information -- List cost and quantity of substrate reagents and supplier Structure Available (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: -- Query Coverage (if not direct match): -- Max Ident (if not direct match): Druggable Target (see Databases for this): Current Inhibitors: Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol or etc): *Amino Acid Sequence: *length of your protein in Amino Acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website Molar Extinction coefficient of your protein at 280 nm wavelength: *CDS Gene Sequence: *GC% Content for gene: *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol: *GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): (link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):
Format for Individual Target pages (copy this list to new Target page and then fill in for your target):
*Target (protein/gene name):*NCBI Gene # or RefSeq#:
*Protein ID (NP or XP #) or Wolbachia#:
*Organism:
Etiologic Risk Group (see link below):
*Background/Disease Information:
Essentiality of this protein:
Complex of enzymes:
*EC#:
Link to BRENDA EC# page:
-- Show screenshot of BRENDA enzyme mechanism schematic
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay or assay reagents (substrates)
-- link to paper that contains assay information
-- List cost and quantity of substrate reagents and supplier
Structure Available (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model:
-- Query Coverage (if not direct match):
-- Max Ident (if not direct match):
Druggable Target (see Databases for this):
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol or etc):
*Amino Acid Sequence:
*length of your protein in Amino Acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
Molar Extinction coefficient of your protein at 280 nm wavelength:
*CDS Gene Sequence:
*GC% Content for gene:
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol:
*GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
(link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):
Resources: